Characterization of T Cell Activation and Function in Cancer Immunotherapy Using High-Throughput Cytometry
Explore the future of cancer immunotherapy with the iQue® Human T Cell Activation Kit — a high-throughput, multiplexed assay platform designed to streamline the evaluation of T cell responses.
T cells are central to the immune system’s ability to recognize and eliminate cancer cells, but solid tumor environments present significant challenges to their efficacy. To support the rapid development of next-generation immunotherapies — including CAR-T cells, bispecific antibodies (BiTEs), and tumor-infiltrating lymphocytes (TILs) — Sartorius introduces a robust solution for comprehensive T cell profiling.
This whitepaper showcases how high-throughput flow cytometry enables simultaneous assessment of multiple parameters — including phenotype, activation status, viability, proliferation, and cytokine secretion — in a single well. Through real-world case studies, the iQue® platform demonstrates:
- Antibody-mediated T cell activation and cytokine profiling to model B-cell leukemia treatments.
- 3D spheroid TIL assays to reveal tumor-specific infiltration dynamics and immune cell phenotypes.
- CAR-T cell assays that uncover antigen-specific activation and “on-target off-tumor” effects in HER2-expressing tumors.
With minimal sample volumes and efficient workflows, researchers can accelerate therapeutic discovery and predict clinical responses more reliably.
Download now to see how Sartorius is enabling fast, actionable insights for immunotherapy development — from bench to bedside.
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