Protein Titer Capabilities – A Comparison of the Cydem VT System to Current Technology across Various CHO Media
In cell line development (CLD), precise IgG quantification is critical for accurate clone ranking. However, traditional offline methods like immunoturbidimetric assays often create operational bottlenecks and introduce risks of human error. This application note evaluates the Cydem VT Automated Clone Screening System—a novel platform that delivers simultaneous, automated daily measurements of both cell health (via trusted Vi-CELL BLU technology) and antibody titer (via fluorescence polarization) from a single sample.
To validate its performance, a 10-point concentration series ($0\text{–}10\text{ g/L}$) was analyzed across various commonly used bioprocessing media formulations, including ActiPro, Freestyle CHO, BalanCD CHO, and PowerCHO 2. The Cydem VT system demonstrated excellent linearity and strong comparability against a market-standard immunoturbidimetric reference device. Furthermore, a 14-day fed-batch culture campaign tracking 10 distinct clones revealed a robust correlation between online and offline titer measurements ($R^2 = 0.972$), ensuring identical clone ranking outcomes.
By streamlining workflows and eliminating manual dilution steps through automatic scaling ($1\times$ to $10\times$), the Cydem VT system offers a highly reproducible, hands-free solution that enhances laboratory efficiency and eliminates the risk of missing high-performing clones.
Key Takeaways
- Dual Functionality: Consolidates automated daily cell health (viability/growth) and IgG titer analysis into a single workflow using the same sample.
- High Correlation: Aligns precisely with traditional immunoturbidimetric methods, delivering a correlation coefficient of $R^2 = 0.972$ during a 14-day fed-batch run.
- Media Robustness: Maintains consistent linearity and accuracy across multiple CHO media environments (ActiPro, Freestyle, BalanCD, and PowerCHO 2).
- Workflow Automation: Minimizes hands-on interaction and human error via automated sampling and an intelligent, multi-step automatic dilution mechanism ($1\times, 2\times, 5\times, 10\times$).
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