Access a world of possibilities with the versatile CytoFLEX platform
Executive Summary
The CytoFLEX Platform by Beckman Coulter Life Sciences delivers flow cytometry from nanoscale to high-dimensional analysis, as well as cell sorting, with exceptional sensitivity and user-friendly operation. Built on semiconductor technology — using Avalanche Photodiode (APD) detectors, a Wavelength Division Multiplexer (WDM), and enhanced optics — the platform maximizes light capture, minimizes spillover due to spreading, and enables detection of dim fluorescent signals across a wide range of applications.
Featuring intuitive CytExpert Software and flexible upgrade paths, the CytoFLEX family allows laboratories to scale from routine 3-laser assays to complex 6-laser, 21-color analyses, or full spectral detection.
Key Products & Core Capabilities
- CytoFLEX & CytoFLEX S Analyzers: Configurable options ranging from 3 to 4 lasers and up to 13 colors for versatile cellular immunophenotyping.
- CytoFLEX LX Flow Cytometer: High-complexity analyzer offering configurations up to 6 lasers and 21 colors.
- CytoFLEX nano Flow Cytometer: Nanoparticle analyzer featuring 6 fluorescent, 5 side scatter, and 1 forward scatter channel — characterizing particles at least as small as 40 nm.*
- CytoFLEX SRT Benchtop Cell Sorter: Benchtop sorter configurable with up to 4 lasers and up to 15-color detection, capable of complex sort logic with up to 4-way sorting.
- CytoFLEX mosaic Spectral Detection Module: Modular add-on for CytoFLEX S* and LX models enabling switching between true conventional and spectral modes, autofluorescence (AF) extraction from up to 10 populations, and a choice of two unmixing algorithms to confirm results quality.
Key Platform Highlights
- APD Sensitivity: High quantum efficiency (QE) detectors support resolution of dim populations and contribute to less data spreading in adjacent detectors compared with PMT-based systems.
- User-Friendly & Streamlined Workflow: CytExpert software offers gain-independent compensation, 21 CFR Part 11 compliance (using CytExpert v2.3 and higher), and fast onboarding — the average user can be trained in under 1 hour.
- Flexible Upgradability: Start with the lasers you need and upgrade later to up to 6 lasers, or add a plate loader or spectral capabilities. Plate loaders are compatible with all CytoFLEX instruments and can be installed without increasing the analyzer’s footprint.
- Cloud-Based Analysis: Access the cloud-based Cytobank platform from CytExpert for Spectral software with a single click for user-friendly, machine learning-powered analysis of complex spectral data.
*Characterized using 40 nm polystyrene beads and triggering on violet side scatter. *Only valid for CytoFLEX S (V-B-Y-R) Series Flow Cytometer.
For Research Use Only. Not for use in diagnostic procedures.
