Achieve Statistically Sound Instrument Harmonization Between Vi-CELL XR and Vi-CELL BLU Cell Viability Analyzers
Reliable cell count and viability measurements are foundational to bioprocess development, scale-up, and manufacturing control. This application note from Beckman Coulter Life Sciences demonstrates a practical, statistically grounded approach for matching Vi-CELL BLU analyzer results to legacy Vi-CELL XR analyzer data.
- Dilutional linearity study designed per ISO 20391-1 and 2, evaluated using the NIST COMET framework across two Vi-CELL XR and two Vi-CELL BLU analyzers
- All devices achieved r² values above 0.999 (CI 95%), low proportionality indices, and mean %CV values of approximately 5% or less at all dilution levels
- Cell type optimization guidance provided, including use of the “Mammalian Enhanced” default cell type (Software version 2.0) for improved cluster counting, debris characterization, and dead cell annotation
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