Thermo Scientific iSC-65 Autosampler and Qtegra ISDS Software: reliability to power through your challenging ICP-OES and ICP-MS workflows
Production of non-natural mRNA transcripts by IVT for vaccines and therapeutics development and manufacturing requires the addition of the modified cap structure post purification. 2 In eukaryotic systems, non-mitochondrial mRNA are capped at the 5’ end by an inverted 7-methylguanosine (m7G) joined to the transcript by a 5’ to 5’ triphosphate linkage, m7G(5’)ppp(5’)N, referred to as Cap(0). 3–5 Once added to the transcript, the first
nucleotide in the strand, (N), is then methylated at the 2’ hydroxyl position to give the Cap(1) structure, m7G(5’)ppp(5’)Nm.
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